Hematological prediction of sepsis and other disease states

G - Physics – 01 – N

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150/11, 167/37

G01N 33/86 (2006.01) G01N 33/50 (2006.01) G01N 33/569 (2006.01) G01N 33/574 (2006.01)

Patent

CA 1226216

ABSTRACT OF THE DISCLOSURE A new diagnostic procedure employing a pre-packaged assay kit to enable the hematological prediction of cancer, sepsis, AIDS and other pathologic states is described. The kits are composed of sterile plastic test tubes and caps wherein a control tube contains an appropriate amount of physio- logic saline, and an activated tube contains various activators of specific pathways of blood coagulation, e.g. endotoxins dissolved in the same volume of saline. Upon the addition of a patient's citrated whole blood (CWB) to each tube, incubation and measurement of recalcification times (RT) are per- formed. A ratio of the control sample RT divided by the activated samples (endotoxin) RT yields a thrombotic index (TI), the values of which are related to various disease states. Low numerical values of this index (if endotoxin is the activating material at 10 µg/cc CWB) indicate post surgical patients at high risk for sepsis (TI µ1.60); values above 1.60 indicate post-surgical patients at low risk for sepsis. Normal volunteers have values ranging from 1.15 - 1.53 whereas newly diagnosed cancer patients have values of 1.62 to 4.39 or higher.

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