Method and/or device for separating motile cells from...

C - Chemistry – Metallurgy – 12 – M

Patent

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195/141, 195/46

C12M 3/00 (2006.01) A61K 35/52 (2006.01) B01L 3/00 (2006.01) G01N 33/50 (2006.01) C12N 5/06 (2006.01)

Patent

CA 1329786

ABSTRACT OF THE DISCLOSURE The invention relates to a device for separating motile cells from less motile, or immotile cells in a mixed cell sample, the device comprising a tubular member which includes a sample loading area, a motile cell collecting area, and an elongated separation area connecting the sample loading area and the cell collecting area, in which the separation area is substantially upright in use, whereby motile cells are able to move from the sample loading area in the separation area through medium placed in the device, into the collecting area, the non motile, or less motile cells being left in the sample loading area and in the separation area. The invention also relates to a method of separating motile cells from non motile, or less motile cells in a mixed cell sample, comprising loading the sample into the sample loading area of a device which contains a suitable medium, ensuring that the separation area of the device is substantially upright, incubating the loaded device for a period of time and collecting pure motile cells from the cell collecting area after the period of incubation.

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