Multi-reporter gene model for toxicological screening

Details Multi-reporter gene model for toxicological screening Multi-reporter gene model for toxicological screening

C - Chemistry, Metallurgy

12

N

C12N 15/62 (2006.01) C07K 14/47 (2006.01) C12Q 1/68 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2531779

A system for the detection of gene activation events is provided which comprises a nucleic acid construct encoding a protein of the lipocalin protein family and a peptide tag in which the expression of the construct in a cell or in the cells of a transgenic animal demonstrates the activation of a gene or genes of interest, in which the protein expressed is secreted from the cell and in which detection of the peptide tag indicates expression of the construct.

L'invention concerne un système de détection d'événements d'activation de gènes qui comprend une construction nucléotidique codant une protéine de la famille de la protéine lipocaline ainsi qu'une étiquette peptidique, dans lequel l'expression de la construction dans une cellule ou dans les cellules d'un animal transgénique démontre l'activation d'un gène ou de gènes étudiés, dans lequel la protéine exprimée est sécrétée par la cellule, et dans lequel la détection de l'étiquette peptidique indique l'expression de la construction.

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