Methods for high resolution gene mapping

Details Methods for high resolution gene mapping Methods for high resolution gene mapping

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2135072

2135072 9322463 PCTABS00027 Two convenient methods for ordering discrete DNA sequences at a high resolution are described. In one embodiment, the invention features a) contacting a eukaryotic cell containng a chromosome of interest with an agent that effects decondensation; b) preparing the cell containing decondensed chromatin for hybridization; and c) hybridizing the cell produced in step b) with at least two DNA probes, each probe being complementary to at least one discrete DNA sequence present on the chromosme of interest; and d) detecting the presence and relative order of the probes as an indication of the relative and absolute locations of the DNA sequences on the chromosome. In another embodiment, the invention features ordering at least two discret DNA sequences on a fragment of DNA that has been extended, for example by physical means, chemical means or a combination of both. A preffered physical means for extending DNA is by gentle smearing.

Affiliated with

Also associated with

Rating

Methods for high resolution gene mapping does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Methods for high resolution gene mapping, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Methods for high resolution gene mapping will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1343417