Method for specific fast detection of relevant bacteria in...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C12Q 1/04 (2006.01)

Patent

CA 2451053

The invention relates to a method for detecting bacteria in drinking water and surface water, especially a method for simultaneous specific detection of bacteria from the Legionella species and the Legionella pneumophila species by in situ-hybridisation. The invention also relates to a method for specific detection of faecal streptococci by in situ-hybridisation and a method for simultaneous specific detection of coliform bacteria and bacteria of the Escherichia coli species, in addition to corresponding oligonucleotide probes and kits enabling said inventive method to be carried out.

L'invention concerne un procédé pour la mise en évidence de bactéries dans l'eau potable et de surface, notamment un procédé pour la mise en évidence spécifique simultanée de bactéries du genreLegionella et de l'espèce Legionella pneumophila par hybridation in situ. L'invention concerne également un procédé pour la mise en évidence spécifique de streptocoques fécaux par hybridation in situ, ainsi qu'un procédé pour la mise en évidence spécifique simultanée de bactéries coliformes et de bactéries de l'espèce Escherichia coli. L'invention concerne en outre des sondes oligonucléotidiques correspondantes, ainsi que des nécessaires permettant de mettre en oeuvre les procédés selon l'invention.

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