Human embryonic lung fibroblast diploid cell strain suitable...

Details Human embryonic lung fibroblast diploid cell strain suitable... Human embryonic lung fibroblast diploid cell strain suitable...

A - Human Necessities

61

K

A61K 39/25 (2006.01) C12N 7/00 (2006.01) C12N 5/08 (2006.01)

Patent

CA 2218358

A human embryonic lung fibroblast diploid cell strain LBHEL (KCTC 0127BP) which is highly susceptible to varicella zoster virus (VZV) is provided. A process for producing a cell-free VZV is provided, comprising the steps of:(a) culturing a human embryonic lung fibroblast diploid cell strain LBHEL in a culture vessel to give cultured LBHEL cells; (b) infecting the cultured LBHEL cells with VZV to give VZV-infected cells; (c)culturing and harvesting the VZV-infected cells; (d) disrupting the harvested cells to obtain a cell homogenate; and (e) centrifuging the cell homogenate to obtain a supernatant containing the cell-free VZV.

L'invention concerne une souche de cellules diploïdes de fibroblastes pulmonaires embryonnaires humaine LBHEL (KCTC 0127BP) très sensible au virus zona-varicelle (VZV). Un virus de VZV exempt de cellules est produit (a) par mis en culture de la souche de cellules diploïdes de fibroblastes pulmonaires embryonnaires humaine LBHEL (KCTC 0127BP) revendiquée dans un récipient de culture pour obtenir une culture de cellules LBHEL; (b) par infection de la culture de cellules LBHEL avec le VZV pour obtenir des cellules infectées par VZV; (c) par culture et recueil des cellules infectées par le VZV; (d) par rupture des cellules recueillies pour obtenir un homogénat de cellules; et (e) par centrifugation de l'homogénat de cellules pour obtenir un surnageant contenant le VZV exempt de cellules.

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