C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/00 (2006.01) C12M 3/00 (2006.01)
Patent
CA 2411524
An apparatus (10) to grow cell cultures includes an anchor (14) potted to a flexible membrane (12) and an anchor stem (16) to which cells attach. A jig (18) having a trough (46) and at least one passage (52) is positioned adjacent the flexible membrane (12). A method for growing and mechanically conditioning three-dimensional cell constructs begins by drawing the flexible membrane (12) and the anchor stem (14) into the trough (46) by means (20) for flexing the flexible membrane (12) (step 114). Cells are supplied (step 116) and allowed to attach to the anchor stem (16) (step 118). The flexible membrane (12) is released from within the trough (46) (step 120), resulting in a three- dimensional structure (34) of cells attached to the anchor stem (16). The cells grow into the three-dimensional construct (step 130). The structure (34) and/or the construct can be subjected to a regimen of strain for mechanical conditioning of the cells (step 132).
L'invention se rapporte à un appareil (10) permettant de faire pousser des cultures cellulaires, et comportant un ancrage (14) rattaché à une membrane flexible (12) et une tige d'ancrage (16) à laquelle les cellules viennent se fixer. Une cuve, (18) pourvue d'un bac (46) et d'au moins une voie de passage (52), est disposée à proximité de la membrane flexible (12). Le procédé permettant de faire pousser et de conditionner mécaniquement des ensembles cellulaires tridimensionnels consiste tout d'abord à disposer la membrane flexible (12) et la tige d'ancrage (14) dans le bac (46) au moyen d'éléments permettant de courber la membrane flexible (12). On utilise des cellules, lesquelles peuvent venir se fixer sur la tige d'ancrage (16). La membrane flexible (12) est retirée du bac (46), laissant ainsi une structure de cellules tridimensionnelle (34) fixée à la tige d'ancrage (16). Les cellules poussent dans l'ensemble tridimensionnel. La structure (34) et/ou l'ensemble peuvent être soumis à un régime de contraintes afin de conditionner mécaniquement les cellules.
Dimock Stratton Llp
Flexcell International Corporation
LandOfFree
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