Plant system for comprehensive gene function analysis with...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01) A01H 1/04 (2006.01) A01H 5/00 (2006.01) C12N 15/10 (2006.01) C12N 15/29 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2458842

A gene causative of a phenotype character is easily specified and thus gene functions are comprehensively analyzed. A method of analyzing a gene function which comprises: (a) infecting a plant mass with a full-length cDNA library containing an expression regulatory sequence to thereby transfer the cDNA into the plants; (b) individual plants fulfilling desired requirements are selected from the plants having the cDNA transferred thereinto as described above; (c) isolating the above-described cDNA from the selected plants; and (d) re- transferring the thus isolated cDNA into a plant of the same species as the transgenic plants as described above to thereby reconfirm the phenotype character depending on the selection requirements as described above.

On peut facilement identifier un gène cause du caractère d'un phénotype et procéder à une analyse approfondie de ses fonctions. L'invention porte sur un procédé d'analyse d'une fonction génique qui comporte les étapes suivantes: (a) infection d'une masse végétale par une bibliothèque d'ADNc contenant une séquence régulatrice d'expression assurant le transfert de l'ADNc dans les plantes; (b) sélection parmi les plantes où l'ADNc a été transféré de plantes individuelles satisfaisant aux exigences requises; (c) isolement dudit ADNc dans les plantes sélectionnées; et (d) retransfert de l'ADNc isolé dans une plante de la même espèce que les susdites plantes transgéniques pour reconfirmer le caractère du phénotype en fonction des exigences de sélection ci-dessus.

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