Method for the isolation and purification of vitamin...

Details Method for the isolation and purification of vitamin... Method for the isolation and purification of vitamin...

C - Chemistry, Metallurgy

12

N

C12N 9/64 (2006.01) A61K 35/16 (2006.01) C07K 1/18 (2006.01) C07K 14/745 (2006.01) C12N 9/74 (2006.01)

Patent

CA 2143510

A method for the separation of vitamin K-dependent proteins from non-vitamin K-dependent accompanying proteins is described wherein the method is characterized in that at least anion exchange chromatography and optionally affinity chromatography is carried out as well. The method is suitable especially for the purification of Factor II, VII, IX, X as well as Protein S, Protein C and Protein Z. With the aid of the method according to the invention a vitamin K-dependent protein is obtained which is present at a purity of 95%.

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