C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 9/24 (2006.01) C12N 1/14 (2006.01) C12P 21/00 (2006.01)
Patent
CA 2245173
A process for the manufacture of a xylanase-rich enzyme complex by the cultivation of a xylanase-producing microorganism of the genus Trichoderma in a nutrient medium comprises using in the cultivation a pre-treated thin stillage of rye as the xylanase inductor and simultaneously as the carbon source, with the pre-treatment comprising a removal of the solid constituents of the thin stillage of rye, a concentration of the non- volatile components by evaporation of water and other volatile substances as well as a subsequent autoclaving of the thin stillage of rye concentrate resulting therefrom. In a further aspect of this process in accordance with the invention , de-oiled soya meal or soya meal liquor is used as an additional xylanase inductor and as a nitrogen source; by the addition of the de-oiled soya meal or soya meal liquor to the pre-treated thin stillage of rye a further increase in the xylanase production is achieved. The enzyme complexes manufactured by the process in accordance with the invention can be used immediately in the animal feedstuff industry, especially in poultry nutrition; their use in, for example, rye-, barley- or triticale-containing feedstuffs has a favourable influence on the reduction of the antinutritive action of the non-starch polysaccharides and leads to an improved digestability and absorption of the nutrients in the intestine of the animal.
Dsm Ip Assets B.v.
F. Hoffmann-La Roche Ag
Gowling Lafleur Henderson Llp
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