C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/70 (2006.01) C12N 1/21 (2006.01) C12N 15/10 (2006.01) C12N 15/63 (2006.01) C12N 15/74 (2006.01) C12N 15/75 (2006.01)
Patent
CA 2483006
The present invention relates to a method of creating promoter cassettes that include modified precursor promoters and transforming a population of bacterial host cells with a promoter library comprising the promoter cassettes resulting in bacterial clones having a range of expression levels of a gene of interest. The invention further relates to selecting a transformed bacterial host cell which has an optimum level of gene expression.
La présente invention concerne une méthode de création de cassettes de promoteurs comprenant des promoteurs précurseurs modifiés, et de transformation d'une population de cellules hôtes bactériennes avec une bibliothèque de promoteurs comprenant des cassettes de promoteurs permettant d'obtenir des clones bactériens présentant une gamme de niveaux d'expression d'un gène d'intérêt. L'invention concerne également la sélection d'une cellule hôte bactérienne transformée avec un niveau optimal d'expression génique.
Cervin Marguerite A.
Valle Fernando
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Genencor International Inc.
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