Selective technique for rapid identification of proteins and...

Details Selective technique for rapid identification of proteins and... Selective technique for rapid identification of proteins and...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C07K 1/22 (2006.01) C12N 15/10 (2006.01)

Patent

CA 2220785

A method relying on synthetic oligonucleotides as ligand for the parallel positive and negative selection for identifying and isolating target molecules that are differentially expressed in a plurality of biological system. These ligands comprise a randomized sequence region flanked by defined and fixed 5' and 3' regions. This method comprises many repetitive steps of incubation of target molecules from two systems with a pool of ligands, followed by a separation and an amplification of the ligands that bind in one system, and do not bind in the other system. The steps are repeated as many times as required to find ligands that bind specifically to the target molecules from system A but do not bind to the target molecules from system B. This method may be used as a very sensitive diagnostic tool since the final pool of ligands can identify target molecules that are different between normal vs pathological systems.

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