Reducing gelation of a fatty acid-acylated protein

C - Chemistry – Metallurgy – 07 – K

Patent

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C07K 14/62 (2006.01) C07K 1/16 (2006.01) C07K 1/34 (2006.01)

Patent

CA 2223531

A method for processing a fatty acid-acylated protein, especially N-palmitoyl LysB29 human insulin, to reduce the incidence of gelation by conducting such processing in the presence of a citrate buffering agent as the primary buffer which method allows such processing to be conducted at higher protein concentrations and with less temperature control than would otherwise be possible in the absence of the citrate buffer.

Méthode pour traiter une protéine acylée par un acide gras, notamment l'insuline humaine N-palmitoyl-LysB29, afin de réduire l'incidence de la gélation, en effectuant ce traitement en présence d'un agent tampon à base de citrate, comme tampon primaire; la méthode permet des traitements de ce type à des concentrations protéiniques plus élevées et avec un contrôle moins strict de la température, comparativement aux possibilités en l'absence de tampon citrate.

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