Methods for detection of a nucleic acid by sequential...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C12N 15/87 (2006.01) C12P 19/34 (2006.01)

Patent

CA 2617815

The invention relates to nucleic acid, flap-mediated, sequential amplification methods which permit detection of a nucleic acid target in a nucleic acid sample. The invention provides for nucleic acid synthesis dependent, flap-mediated amplification methods for sequentially producing detectable, released flaps to detect a target nucleic acid. The invention provides for both linear, and exponential nucleic acid synthesis dependent, flap-mediated, sequential amplification methods.

L'invention concerne des procédés d'amplification séquentielle facilitée par flap de l'acide nucléique, ces procédés permettant la détection d'une cible d'acide nucléique dans un échantillon d'acide nucléique. La présente invention porte également sur des procédés d'amplification facilitée par flap et dépendante de la synthèse d'acide nucléique pour produire de manière séquentielle des flaps libérés pouvant être détectés, afin de détecter un acide nucléique cible. L'invention concerne aussi des procédés d'amplification séquentielle facilitée par flap et dépendante de la synthèse d'acide nucléique, à la fois linéaire et exponentielle.

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