Cell culture method

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 5/10 (2006.01) A61K 35/30 (2006.01) C12N 5/06 (2006.01) C12N 15/52 (2006.01) C12N 15/65 (2006.01) A61K 35/12 (2006.01)

Patent

CA 2214385

A method for selectively culturing a pre-selected sub-population of cells from a heterogeneous cell population in vitro, comprises the steps of: (a) introducing a selectable marker (e.g. a positive and/or negative selectable marker) into the heterogeneous cell population, which marker is subject to differential expression/activity in the pre-selected sub-population; and (b) selectively culturing the pre-selected sub-population on the basis of the differential expression/activity therein of the selectable marker.

Cette invention concerne un procédé permettant la culture sélective in vitro d'une sous-population présélectionnée de cellules provenant d'une population hétérogène de cellules. Ce procédé consiste (a) à introduire un marqueur sélectionnable (par exemple positif et/ou négatif) au sein de la population hétérogène de cellules, ledit marqueur étant soumis à une activité ou à une expression différentielle en présence de la sous-population, et (b) à cultiver de manière sélective la sous-population en fonction de l'activité ou de l'expression différentielle du marqueur sélectionnable au sein de ladite population.

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