Apparatus including ion transport detecting structures and...

G - Physics – 01 – N

Patent

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G01N 1/00 (2006.01) B01L 3/00 (2006.01) B03C 5/02 (2006.01) C12N 9/34 (2006.01) G01N 15/10 (2006.01) G01N 27/26 (2006.01) G01N 27/447 (2006.01) G01N 33/487 (2006.01) G01N 33/50 (2006.01) B81B 1/00 (2006.01)

Patent

CA 2485099

The present invention recognizes that the determination of ion transport function or properties using direct detection methods, such as whole cell recording or single channel recording, are preferable to methods that utilize indirect detection methods, such as FRET based detection system. The present invention provides biochips and other fluidic components and methods of use that allow for the direct analysis of ion transport function or properties using microfabricated structures that can allow for automated detection of ion transport function or properties. These biochips and fluidic components and methods of use thereof are particularly appropriate for automating the detection of ion transport function or properties, particularly for screening purposes.

La présente invention reconnaît que la détermination de la fonction ou des propriétés de transport d'ions au moyen de processus de détection directe, tels que l'enregistrement de cellules entières ou l'enregistrement de canaux uniques, sont préférables aux procédés qui utilisent des processus de détection indirecte tels que le système de détection basé sur le transfert d'énergie de résonance par fluorescence. La présente invention a trait à des biopuces et d'autres composants fluidiques et des procédés d'utilisation qui permettent l'analyse directe de la fonction ou des propriétés de transport d'ions ou au moyen de structures micro-fabriquées qui permettent la détection automatique de la fonction et des propriétés de transport d'ions. Ces biopuces et composants fluidiques et les procédés d'utilisation sont particulièrement appropriés pour l'automatisation de la détection, notamment à des fins de criblage.

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