Scaffold-free self-organized 3d synthetic tissue

Details Scaffold-free self-organized 3d synthetic tissue Scaffold-free self-organized 3d synthetic tissue

C - Chemistry, Metallurgy

12

N

C12N 5/071 (2010.01) A61K 35/12 (2006.01) A61L 27/38 (2006.01)

Patent

CA 2534356

The present invention can be used for actual implantation surgery without a scaffold. The present invention provides a synthetic tissue or complex which can be produced by culture and has a high level of differentiation ability. The present invention also provides a therapy and medicament for repairing and/or regenerating tissue using replacement and covering. By culturing cells under specific culture conditions such that medium contains an extracellular matrix synthesis promoting agent, the cells are organized and are easily detached from a culture dish. The present invention was achieved by finding such a phenomenon. In addition, the self contraction of the tissue can be regulated by culturing the tissue in a suspended manner. Therefore, it is possible to regulate the three-dimensional shape of the tissue. The present invention also provides a method for producing an implantable synthetic tissue which does not require a plurality of monolayer cell sheets assembled to form a three-dimensionally structured synthetic tissue. The present invention is characterized by richness in adhesion molecules, nonnecessity of additional fixation at an implantation site, and good biological integration.

La présente invention peut être utilisée en chirurgie d'implantation sans structure. La présente invention concerne un tissu ou complexe synthétique pouvant être obtenu par mise en culture et avec un degré élevé de capacité de différenciation. La présente invention concerne également une thérapie et un médicament de réparation et/ou de régénération d'un tissu par substitution et recouvrement. La mise en culture de cellules dans des conditions de culture spécifiques, grâce auxquelles le milieu peut contenir un agent favorisant la synthèse matricielle extracellulaire, permet d'organiser les cellules et de les retirer facilement d'une boîte de Pétri, ce qui a permis de mettre au point la présente invention. De plus, l'auto-contraction du tissu peut être régulée par mise en culture du tissu en suspension. Il est ainsi possible de réguler la forme tridimensionnelle du tissu. La présente invention concerne en outre une méthode de production d'un tissu synthétique implantable ne nécessitant pas une pluralité de feuilles cellulaires monocouche assemblées pour former un tissu synthétique structuré de manière tridimensionnelle. La présente invention se caractérise par sa richesse en molécules d'adhésion, par la non nécessité d'une fixation supplémentaire au niveau du site d'implantation, et par une bonne intégration biologique.

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