A - Human Necessities – 01 – H
Patent
A - Human Necessities
01
H
A01H 4/00 (2006.01) A01C 1/02 (2006.01) A01H 7/00 (2006.01) C12N 5/04 (2006.01) C12Q 1/00 (2006.01) G01N 33/50 (2006.01) G01N 33/53 (2006.01) G01N 33/68 (2006.01)
Patent
CA 2284089
The invention concerns measurement of sucrose series oligosaccharides, particularly sucrose, raffinose, and stachyose, and the dehydrin group proteins, in conifer somatic embryos as a measure of their biochemical maturity and readiness to germinate. The information gained is useful for evaluation of the effectiveness of the culture media, particularly that used for the development of the cotyledonary embryo stage. Somatic embryos having elevated levels of the oligosaccharides have been shown to have improved germination, especially those in the genus Pinus. The invention is also directed to conifer somatic embryos having elevated levels of sucrose series oligosaccharides and dehydrin group proteins.
L'invention concerne la mesure du taux d'oligosaccharides de la série des saccharoses, notamment le saccharose, le raffinose, le stachyose et des protéines du groupe de la déhydrine, dans les embryons somatiques de conifères, pour mesurer leur maturité biochimique et leur état de préparation à la germination. Les informations recueillies sont utiles pour l'évaluation de l'efficacité des milieux de culture, notamment ceux utilisés pour le développement de la phase embryonnaire cotylédonaire. Les embryons somatiques ayant des taux élevés d'oligosaccharides se sont avérés présenter une germination améliorées, notamment ceux du genre Pinus. L'invention se rapporte également à des embryons somatiques de conifères, ayant des taux élevés d'oligosaccharides de la série des saccharoses et de protéines du groupe de la déhydrine.
Blackman Sheila A.
Carpenter Carolyn V.
Gupta Pramod K.
Koester Martha K.
Smart & Biggar
Weyerhaeuser Company
Weyerhaeuser Nr Company
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