G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/53 (2006.01) C07K 16/18 (2006.01) G01N 33/577 (2006.01) G01N 33/68 (2006.01)
Patent
CA 2506202
An immunoassay method for conveniently and highly accurately assaying an elastin digestion product in a circulatory fluid of a subject which is useful in detecting aortic dissection. This method, which comprises immunologically binding a first antibody and a second antibody to an elastin digestion product, is characterized in that the first antibody and the second antibody are each an antibody selected from the group consisting of monoclonal antibodies produced by a hybridoma HASG-30 (FERM BP-08489), a hybridoma HASG-2 (FERM BP-08488) and a hybridoma HASG-61-1 (FERM BP-08490) and antibodies having specificity and affinity for the human aortic elastin digestion product equivalent to any of these monoclonal antibodies.
L'invention concerne un dosage immunologique permettant de doser de manière appropriée et extrêmement précise un produit de la digestion de l'élastine dans un fluide de circulation d'un sujet, ce qui est utile dans la détection de la dissection de l'aorte. Cette méthode qui comprend la liaison immunologique d'un premier anticorps et d'un second anticorps à un produit de la digestion de l'élastine est caractérisée en ce que le premier anticorps et le second anticorps constituent respectivement un anticorps sélectionné parmi le groupe comprenant des anticorps monoclonaux produits par un hybridome HASG-30 (FERM BP-08489), un hybridome HASG-2(FERM BP-08488) et un hybridome HASG-61-1 (FERM BP-08490), et des anticorps possédant une spécificité et une affinité au produit de la digestion de l'élastine de l'aorte humaine correspondant à n'importe lequel de ces anticorps monoclonaux.
Eisai Co. Ltd.
Ogilvy Renault Llp/s.e.n.c.r.l.,s.r.l.
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