Constituent layer harvesting from a centrifuged sample in a...

Details Constituent layer harvesting from a centrifuged sample in a... Constituent layer harvesting from a centrifuged sample in a...

G - Physics

01

N

G01N 33/49 (2006.01) B01L 3/14 (2006.01) G01N 1/10 (2006.01)

Patent

CA 2058670

INVENTORS: Robert A. Levine Stephen C. Wardlaw TITLE: "CONSTITUENT LAYER HARVESTING FROM A CENTRIFUGED SAMPLE IN A TUBE" DOCKET NO.: H-1170 ABSTRACT OF THE DISCLOSURE Constituent layers are harvested from a centrifuged multi-constituent material in an evacuated glass or clear plastic tube which contains a float. When possibly contaminated materials, such as blood, are being tested, the use of an evacuated tube allows the measurements to be made without the technician being exposed to the blood. The tubes are large enough to hold approximately one ml of blood, and arefilled with an inert gas at low pressure. The floats are formed with a through bore into which cell bands to be harvested will settle during centrifugation. The cell bands are stabilized by a layer of a flowable material which settles onto the plasma layer during centrifugation and forms a pellicle thereon. The cell layers to be harvested areaspirated from the float bore by means of a hypodermic needle or cannula inserted into the tube and float bore.

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