Megakaryocyte colony stimulating factor and production thereof

Details Megakaryocyte colony stimulating factor and production thereof Megakaryocyte colony stimulating factor and production thereof

C - Chemistry, Metallurgy

12

P

C12P 21/00 (2006.01) C07K 14/53 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2084074

(57) Abstract A homogeneous megakaryocyte colony stimulating factor (Meg-CSF) comprising a protein having an activity of forming a megakaryocyte colony from human or mouse myelloid cells in vitro and an activity of increasing the numbers Or megakaryocyte precursor cells and megakaryocytes in vivo, said Meg-CSF being produced by culturing cell strains of a large human-derived pul- monary cell carcinoma, e.g. PC-13 strains, or human pulmonary carcinoma cell strains, i.e. MC-1 strains, separated as monoclon- al cells from the PC-13 strains, recovering the supernatant of the resulting culture medium, separating the Meg-CSF from the su- pernatant, and purifying it by a newly developed purification technique. The obtained homogeneous Meg-CSF is a homogeneous protein having a molecular weight of about 23,000 according to gel filtration or about 20,000 according to SDS-PAGE and an isoelectric point of 4.5 to 5.5. The partial amino acid sequence of the protein has been determined. The homogeneous Meg-CSF is useful for treating a certain kind of thrombocytopenia.

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