C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
C12P 19/34 (2006.01) C12N 15/10 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2695264
The present invention is directed to methods, compositions, software and devices for enriching low abundance alleles from a sample. The method is based in part on a modified nucleic acid amplification protocol that includes incubating the reaction mixture at a critical denaturing temperature or "Tc". By employing the present invention the current detection limits of all PCR-based technologies are greatly improved.
La présente invention porte sur des procédés, des compositions, un logiciel et des dispositifs permettant d'enrichir des allèles à abondance faible provenant d'un échantillon. Le procédé repose en partie sur un protocole d'amplification d'acide nucléique modifié et consiste à incuber le mélange réactionnel à une température de dénaturation critique ou = Tc =. La présente invention permet d'améliorer grandement les limites de détection courantes de toutes les technologies reposant sur une PCR.
Borden Ladner Gervais Llp
Dana-Farber Cancer Institute
LandOfFree
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