Rna polymerase

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/54 (2006.01) C12N 1/21 (2006.01) C12N 9/12 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2265884

An RNA polymerase comprising a wild type RNA polymerase with at least one amino acid modified so as to have a higher ability of incorporating 3'- deoxyribonucleotide or a derivative thereof than that of the corresponding wild type RNA polymerase. Specifically, for example, an RNA polymerase comprising a wild type RNA polymerase wherein at least one amino acid present in the nucleotide bonding site, for example, phenylalanine, is substituted by tyrosine. The RNA polymerase has little or no bias of incorporation between ribonucleotide and 3'-deoxyribonucleotide, between ribonucleotides having different bases, and between deoxyribonucleotides having different bases.

Cette ARN polymérase renferme une polymérase de type sauvage possédant au moins un acide aminé modifié de manière à être plus à même d'incorporer un 3'-désoxyribonucléotide ou l'un de ses dérivés que l'ARN polymérase de type sauvage correspondante. L'invention a trait, plus précisément, à une ARN polymérase renfermant une ARN polymérase dans laquelle un amino acide, un à tout le moins, présent dans le site de fixation du nucléotide, une phénylalanine par exemple, est substitué par de la tyrosine. Cette ARN polymérase ne présente qu'une faible tendance, sinon pas du tout, à l'incorporation entre un ribonucléotide et un 3'-désoxyribonucléotide, entre des ribonucléotides à différentes bases ainsi qu'entre des désoxyribonucléotides à différentes bases.

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