Pesticidal proteins and methods for their use

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/82 (2006.01) C07K 14/325 (2006.01)

Patent

CA 2753491

Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:50-96, or the nucleotide sequence set forth in SEQ ID NO: 1-47, as well as variants and fragments thereof.

La présente invention concerne des compositions et des procédés pour conférer une activité pesticide à des bactéries, des plantes, des cellules végétales, des tissus et des graines. L'invention concerne des compositions comprenant une séquence codante d'un polypeptide toxine. Les séquences codantes peuvent être utilisées dans des produits de recombinaison d'ADN ou dans des cassettes d'expression pour la transformation et l'expression dans les plantes et les bactéries. Les compositions comprennent également des bactéries, des plantes, des cellules végétales, des tissus et des graines transformés. En particulier, l'invention concerne des molécules d'acide nucléique de toxine. De plus, l'invention concerne des séquences d'acides aminés correspondant aux polynucléotides, ainsi que des anticorps se liant spécifiquement à ces séquences d'acides aminés. En particulier, la présente invention concerne des molécules d'acide nucléique isolées comprenant des séquences nucléotidiques codant pour la séquence d'acide amine montrée dans SEQ ID n° 50-96, ou la séquence nucléotidique décrite dans SEQ ID n° 1-47, ainsi que leurs variants et fragments.

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