Monoclonal antibody to acetylated lysine residues in...

Details Monoclonal antibody to acetylated lysine residues in... Monoclonal antibody to acetylated lysine residues in...

C - Chemistry, Metallurgy

12

N

C12N 5/18 (2006.01) C07K 14/575 (2006.01) C07K 14/61 (2006.01) C07K 16/26 (2006.01) G01N 33/577 (2006.01) G01N 33/74 (2006.01)

Patent

CA 2062959

Provided are hybridomas for producing monoclonal antibodies against acetylated lysine residues of rbSt. The antibodies are specific to the presence of at least a single acetylated lysine in a protein and can differentiate between .alpha.-acetyl and .epsilon.-acetyl lysine as free amino acids. The invention provides a guantitative and efficient test for acetylated impurities in recombinant proteins prepared using E.coli as the host.

On prévoit des hybridomes afin de produire des anticorps monoclonaux dressés contre les résidus de lysine acétylée de la somatotropine bovine recombinante. Les anticorps sont spécifiques à la présence d'au moins une seule lysine acétylée dans une protéine et peuvent différencier entre la lysine alpha-acétyle et la lysine epsilon-acétyle en tant qu'aminoacides libres.

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