Production of closed linear dna

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01)

Patent

CA 2751130

An in vitro process for the production of closed linear deoxyribonucleic acid (DNA) comprises (a) contacting a DNA template comprising at least one protelomerase target sequence with at least one DNA polymerase in the presence of one or more primers under conditions promoting amplification of said template; and (b) contacting amplified DNA produced in (a) with at least one protelomerase under conditions promoting production of closed linear DNA. A kit provides components necessary in the process.

La présente invention porte sur un procédé in vitro permettant la production d'un acide désoxyribonucléique (ADN) linéaire fermé comprenant (a) la mise en contact d'une matrice ADN comprenant au moins une séquence de protélomérase cible avec au moins une ADN polymérase en présence d'une ou plusieurs amorces dans des conditions favorisant l'amplification de ladite matrice; et (b) la mise en contact de l'ADN amplifié produit en (a) avec au moins une protélomérase dans des conditions favorisant la production d'ADN linéaire fermé. L'invention porte également sur une trousse contenant des composants nécessaires au procédé.

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