Use of denaturing agents during affinity capture

Details Use of denaturing agents during affinity capture Use of denaturing agents during affinity capture

G - Physics

01

N

G01N 33/543 (2006.01)

Patent

CA 2657223

A method for affinity capturing target molecules is disclosed, comprising obtaining a sample comprising a target molecule; and, in the presence of a denaturing agent; affinity capturing the target molecule with a single chain affinity molecule or antigen binding portion thereof that specifically binds the target molecule, wherein the affinity molecule or antigen binding portion thereof is immobilized on a support.

L'invention concerne un procédé de capture par affinité de molécules cibles qui comprend l'obtention d'un échantillon comprenant une molécule cible ; et, en présence d'un agent de dénaturation,la capture par affinité de la molécule cible avec une molécule d'affinité à chaîne unique ou une partie de celle-ci se liant à un antigène qui se lie de façon spécifique à la molécule cible, où la molécule d'affinité ou la partie de celle-ci se liant à un antigène est immobilisée sur un support.

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