Method for generating stable cell lines expressing high...

Details Method for generating stable cell lines expressing high... Method for generating stable cell lines expressing high...

C - Chemistry, Metallurgy

12

N

C12N 15/65 (2006.01) C12N 15/67 (2006.01) C12N 15/79 (2006.01)

Patent

CA 2690939

This invention relates to industrial production of proteins. More specifically, the invention relates to a method for obtaining cells that stably express a protein of interest, even when cultivated in the absence of selective pressure. DHFR is used as a surrogate marker. The transfected cells are not selected based on resistance to a toxic compound, but based on fluorescence as measured by FACS using fluorescent MTX.

Cette invention concerne la production industrielle de protéines. Plus précisément, l'invention porte sur un procédé d'obtention de cellules qui expriment de façon stable une protéine d'intérêt, même lorsqu'elles sont cultivées en l'absence d'une pression sélective. La DFHR est utilisée comme substitut de marqueur. Les cellules transfectées ne sont pas sélectionnées sur la base de la résistance à un composé toxique, mais sur la base d'une fluorescence telle que mesurée par une FACS utilisant un MTX fluorescent.

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