Electrical impedance tomography to control electroporation

C - Chemistry – Metallurgy – 12 – N

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C12N 13/00 (2006.01) A61B 5/053 (2006.01) C12M 3/00 (2006.01) G01N 33/487 (2006.01)

Patent

CA 2378147

Images created by electrical impedance tomography (EIT) are used to adjust one or more electrical parameters and obtain a desired degree of electroporation of cells in tissue. The parameters include current, voltage and a combination thereof. The cells are subjected to conditions such that they become permeabilized but are preferably not subjected to conditions which result in irreversible pore formation and cell death. The electroporation can analyze cell membranes, diagnose tissues and the patient as well as to move materials into and out of cells in a controlled manner.

L'invention concerne des images créées par tomographie d'impédance électrique (TIE), utilisées pour régler au moins un paramètre électrique et obtenir un degré voulu d'électroporation de cellules dans un tissu. Les paramètres comprennent notamment le courant, la tension et leur combinaison. Les cellules sont soumises à des conditions telles qu'elles deviennent perméables mais, de préférence, non à des conditions qui entraîneraient une formation de pores irréversible et la mort de cellules. L'électroporation permet d'analyser les membranes cellulaires, de diagnostiquer les tissus et le patient ainsi que de déplacer des matières à l'intérieur et hors des cellules avec maîtrise.

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