Exonuclease mediated strand displacement amplification

Details Exonuclease mediated strand displacement amplification Exonuclease mediated strand displacement amplification

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2058567

P-1763 EXONUCLEASE MEDIATED STRAND DISPLACEMENT AMPLIFICATION Abstract This invention relates a nucleic acid target amplification method which operates at a single temperature and makes use of a polymerase in conjunction with an exonuclease that will digest the primer portion of the polymerized strand such that the polymerase will displace the partially digested strand without digestion of the target strand while generating a newly polymerized strand.

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