Transcription control factor zhx3

C - Chemistry – Metallurgy – 07 – K

Patent

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C07K 14/47 (2006.01) A61K 38/45 (2006.01) A61K 45/00 (2006.01) A61P 1/16 (2006.01) A61P 35/00 (2006.01) C07K 16/18 (2006.01) C12N 15/12 (2006.01) G01N 33/15 (2006.01) G01N 33/50 (2006.01) G01N 33/53 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2499354

To determine the biological role of ZHX1, found previously, which acts as a transcriptional repressor, the inventors conducted a search of ZHX1 - interacting proteins using a yeast two-hybrid system. Molecular cloning and determination of the nucleotide sequence of the full- length cDNA encoding a novel protein revealed a novel protein with SEQ ID NO: 1. The protein (ZHX3), like ZHX1, contains two zinc-finger (Znf) motifs and five homeodomains (HDs) and has a transcriptional repressor activity.

On a recherché, à l'aide d'un système hybride de levure 2, une protéine qui interagit avec ZHX1, afin de déterminer le rôle biologique de ZHX1 trouvée auparavant et son fonctionnement en tant que répresseur de transcription. Il a résulté de cette recherche qu'on a trouvé une nouvelle protéine de numéro de séquence 1 lors de la détermination d'un clonage moléculaire d'ADNc entier codant la nouvelle protéine et une séquence nucléotidique de cette dernière. Il est apparu clairement que cette protéine (ZHX3), comme ZHX1, contient deux motifs en doigts de zinc (Znf) et cinq homéodomaines (HD) et présente une activité inhibant la transcription.

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