C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/29 (2006.01) C07K 14/43 (2006.01) C12N 1/19 (2006.01) C12N 15/62 (2006.01) C12N 15/81 (2006.01)
Patent
CA 2364005
The present invention relates to a single-chain monellin-like protein which is stable and which is at least 100-fold sweet as compared to sucrose on the weight basis. The present invention also relates to a nucleic acid encoding said monellin-like protein. Preferably, the nucleic acid further comprises a promoter and a signal sequence for directing expression and secretion of the encoded monellin-like protein in the methylothrophic yeast Pichia pastoris. The present invention further relates to a recombinant Pichia pastoris cell containing the nucleic acid encoding the monellin-like protein, a process for producing the monellin-like protein from the recombinant Pichia pastoris and product of the process.
L'invention concerne une protéine du type monelline, à une seule chaîne, stable et qui, à poids égal, est 100 fois plus sucrée que le sucrose. L'invention concerne également un acide nucléique codant cette protéine du type monelline. De préférence, l'acide nucléique comprend en outre un promoteur ainsi qu'une séquence signal destinée à diriger l'expression et la sécrétion de la protéine codée du type monelline, dans la levure méthylotrophe Pichia pastoris. L'invention concerne enfin une cellule Pichia pastoris recombinée contenant l'acide nucléique codant la protéine du type monelline, un procédé de production de la protéine du type monelline, à partir de Pichia pastoris recombinée, ainsi que le produit obtenu à l'aide de ce procédé.
Fetherstonhaugh & Co.
Genway Biotech Inc.
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