Aqueous multiple-phase isolation of polypeptide

Details Aqueous multiple-phase isolation of polypeptide Aqueous multiple-phase isolation of polypeptide

C - Chemistry, Metallurgy

12

N

C12N 15/18 (2006.01) C07K 1/113 (2006.01) C07K 14/61 (2006.01) C07K 14/65 (2006.01)

Patent

CA 2167910

A method is described for isolating an exogenous polypeptide in a non-native conformation from cells, such as an aqueous fermentation broth, in which it is prepared comprising contacting the polypeptide with a chaotropic agent and preferably a reducing agent and with phase--forming species to form multiple aqueous phases, with one of the phases being enriched in the polypeptide and depleted in the biomass solids and nucleic acids originating from the cells. Preferably, the method results in two aqueous phases, with the upper phase being enriched in the polypeptide.

Méthode d'isolement d'un polypeptide exogène dans une conformation non native de cellules, telle qu'un bouillon de culture aqueux, qu'on prépare en mettant en présence le polypeptide et un agent chaotrope et de préférence un agent réducteur et une espèce génératrice de phases aqueuses multiples, dont l'une est enrichie en polypeptides et appauvrie en solides provenant de la biomasse et en acide nucléique provenant des cellules. La méthode comporte de préférence deux phases aqueuses dont la phase supérieure est enrichie en polypeptides.

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