C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/0797 (2010.01) A61K 35/30 (2006.01) A61P 25/00 (2006.01) A61K 35/12 (2006.01)
Patent
CA 2507395
A medium containing hepatocyte growth factor (HGF) was shown to induce neurosphere formation. Furthermore, the addition of HGF to a culture medium containing FGF-2, EGF, or both increased both the size and number of newly formed neurospheres. Thus, the present invention relates to a growth medium comprising HGF for culturing neural stem cells and methods for culturing the cells using the culture medium.
L'invention concerne un milieu comprenant le facteur de croissance hépatocyte (HGF) induisant une formation de neurosphères. De plus, l'ajout du facteur HGF dans un milieu de culture renfermant FGF-2 et/ou EGF permet d'accroître aussi bien les dimensions que le nombre des neurosphères nouvellement formées. Par conséquent, l'invention concerne un milieu de croissance renfermant le facteur HGF, aux fins de culture de cellules souches neurales et des procédés de culture des cellules au moyen du milieu de culture.
Iwama Toru
Kaku Yasuhiko
Kitajima Hideomi
Kokuzawa Jouji
Kunisada Takahiro
Anges Mg Inc.
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
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