Method for producing insulin analogs having a dibasic b...

Details Method for producing insulin analogs having a dibasic b... Method for producing insulin analogs having a dibasic b...

Category

C - Chemistry, Metallurgy

Subcategory

07

Subsubcategory

K

IPC codes

C07K 14/62 (2006.01) C12P 21/02 (2006.01)

Type

Patent

Patent number

CA 2657041

Description

The invention relates to a method for producing a type of insulin by genetically engineering a precursor thereof and converting said precursor to the respective insulin in an enzyme-catalyzed ligation reaction with lysine amide or arginine amide, or by lysine or arginine which is modified by protective groups, and optionally subsequent hydrolysis.

L'invention concerne un procédé servant à produire une insuline. Selon l'invention, on produit par biotechnologie un précurseur de cette insuline que l'on fait ensuite réagir avec un amide de lysine ou un amide d'arginine ou avec de la lysine ou de l'arginine modifiée par des groupes protecteurs lors d'une réaction de ligature catalysée par enzymes, éventuellement suivie d'une hydrolyse, afin d'obtenir cette insuline.

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