High sensitivity protein workstation and techniques

G - Physics – 01 – N

Patent

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Details

G01N 35/10 (2006.01) B01L 3/00 (2006.01) B01L 3/02 (2006.01) C12Q 1/68 (2006.01) G01N 27/447 (2006.01) G01N 33/53 (2006.01) G01N 35/02 (2006.01) G01N 1/34 (2006.01) G01N 1/40 (2006.01) G01N 35/00 (2006.01)

Patent

CA 2469937

A method of selecting and identifying bio-molecules present in a bio-sample is disclosed. The method comprises the steps of: obtaining a bio-sample; amplifying (2) the bio-molecules present in the bio-sample to improve the ease of detection of said bio-molecules; separating the bio-molecules in said amplified bio-sample; depositing (3) the amplified bio-molecules on to a suitable media. Detecting means are then used to identify or detect the presence of bio-molecules in said amplified and separated sample wherein said amplification step occurs in close physical proximity to said deposition step. A device (1) for carrying out the method is also disclosed as is a protein chip library produced by the device or method.

L'invention concerne un procédé de sélection et d'identification de biomolécules présentes dans un échantillon biologique. Le procédé comprend les étapes consistant: à obtenir un échantillon biologique; à amplifier (2) les biomolécules présentes dans l'échantillon biologique, afin de faciliter la détection desdites biomolécules; à séparer les biomolécules dans l'échantillon biologique amplifié; à déposer (3) les biomolécules amplifiées sur un support approprié. Des moyens de détection sont ensuite utilisés pour identifier ou détecter la présence de biomolécules dans l'échantillon amplifié et séparé, l'étape d'amplification ayant lieu à une proximité physique très étroite de l'étape de dépôt. L'invention concerne également un dispositif (1) permettant de mettre en oeuvre le procédé, ainsi qu'une banque de puce protéomique obtenue au moyen du dispositif ou du procédé.

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