Oxygen-assisted fermentation process

C - Chemistry – Metallurgy – 12 – M

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C12M 1/04 (2006.01) B01F 3/04 (2006.01) C12M 1/36 (2006.01)

Patent

CA 2556679

A fermentation process uses substantially pure oxygen. The oxygen is the only reactive gas which is injected into a fermentation vessel. The oxygen is moved through the vessel solely by its own pressure. The process can be used with both mechanically-agitated and air-lifted fermenters. The mechanically- agitated fermenter includes an analyzer (35) for measuring oxygen concentration in the exhaust line, and adjusting the flow of fresh oxygen into the vessel accordingly. In the air-lifted fermenter, an analyzer measures the oxygen concentration in the head space of the vessel, and operates valves which either recycle the gas from the head space, or vent that gas to the outside, according to the measured concentration. A stream of nitrogen is periodically injected into the vessel to drive out carbon dioxide and other gases, to control the pH of the fermentation medium. The present invention substantially improves the efficiency of a commercial fermentation process.

Un processus de fermentation utilise sensiblement de l'oxygène pur. L'oxygène est le seul gaz réactif qui est injecté dans une cuve de fermentation. L'oxygène se déplace dans la cuve uniquement grâce à sa propre pression. Le procédé peut s'utiliser soit avec des dispositifs de fermentation à agitation mécanique soit à l'air qui monte. Le dispositif de fermentation à agitation mécanique comprend un analyseur (35) destiné à mesurer la concentration d'oxygène dans la ligne d'échappement et à ajuster le flux d'oxygène frais dans la cuve, respectivement. Dans le dispositif de fermentation à l'air qui monte, un analyseur mesure la concentration d'oxygène dans l'espace supérieur de la cuve et actionne des vannes qui soit recyclent le gaz provenant de l'espace supérieur soit éjectent le gaz vers l'extérieur, en fonction de la concentration mesurée. Un flux d'azote est périodiquement injecté dans la cuve de manière à repousser le dioxyde de carbone et d'autres gaz, pour réguler le pH du milieu de fermentation. La présente invention permet d'améliorer sensiblement l'efficacité d'un processus de fermentation commercial.

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