Production of proteins using homologous recombination

Details Production of proteins using homologous recombination Production of proteins using homologous recombination

C - Chemistry, Metallurgy

12

P

C12P 21/00 (2006.01) C07K 14/505 (2006.01) C12N 5/10 (2006.01) C12N 15/85 (2006.01) C12N 15/86 (2006.01)

Patent

CA 2045175

Methods and compositions are provided for expression of mammalian genes in culture. An amplifiable gene is introduced by homologous recombination in juxtaposition to a target gene, the resulting combination of amplifiable gene and target gene transferred to a convenient host and the target gene amplified by means of the amplifiable gene. The resulting expression host may then be grown in culture with enhanced expression of the target gene.

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