Modified gene-silencing rna and uses thereof

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/11 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2478904

Methods and means for efficiently downregulating the expression of any gene of interest in eukaryotic cells and organisms are provided. To this end, the invention provides modified antisense and sense RNA molecules, chimeric genes encoding such modified antisense or sense RNA molecules and eukaryotic organisms such as plants, animals or fungi, yeast or molds comprising the modified antisense and/or sense RNA molecules or the encoding chimeric genes.

L'invention concerne des procédés et des moyens permettant de réguler négativement et efficacement l'expression d'un gène étudié quelconque dans des cellules et des organismes eucaryotes. Afin d'atteindre ce but, l'invention concerne des molécules d'ARN anti-sens et sens modifiées, des gènes chimères codant de telles molécules et des organismes eucaryotes, tels que des végétaux, des animaux ou des champignons, de la levure ou des moisissures comprenant les molécules d'ARN anti-sens et/ou sens modifiées ou les gènes chimères de codage.

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