C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/10 (2006.01) C12N 9/56 (2006.01) C12Q 1/68 (2006.01) C07B 61/00 (2006.01)
Patent
CA 2426774
Methods are disclosed for producing libraries of nucleic acid molecules which libraries are derived from a nucleic acid template. The libraries comprise variant nucleic acids which are produced from a mutagenesis strategy using, e.g., a plurality of defined mutagenic and/or non-mutagenic primers and specific reaction conditions which favor the production of varied combinatorial mutants.
L'invention concerne des procédés de production de bibliothèques de molécules d'acide nucléique, ces bibliothèques étant dérivées d'une matrice d'acide nucléique. Les bibliothèques comprennent des acides nucléiques variants produits à partir d'une stratégie de mutagenèse à l'aide, par exemple, d'une pluralité d'amorces mutagéniques et/ou non mutagéniques définies, et de conditions de réaction spécifiques favorisant la production de mutants combinatoires variés.
Caldwell Robert M.
Schellenberger Volker
Bereskin & Parr
Genencor International Inc.
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