Separations platform based upon electroosmosis-driven planar...

B - Operations – Transporting – 01 – D

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B01D 15/08 (2006.01)

Patent

CA 2558305

The present invention describes a system and method for separation of proteins, peptides and glycans by one-dimensional or two-dimensional electroosmosis-driven planar chromatography. Separation is performed using amphiphilic polymeric membranes, amphiphilic thin-layer chromatography plates or other planar amphiphilic surfaces as the stationary phase with a combination of organic and/or aqueous buffers as the mobile phase. Systematic selection of stationary phase supports, mobile phase buffers and operating conditions allow for the adaptation of the invention to a broad range of applications in proteomics, mass spectrometry, drug discovery and the pharmaceutical sciences.

La présente invention concerne un système et un procédé pour séparer des protéines, des peptides et des glycanes grâce à une chromatographie de surface par électro-osmose à une dimension ou à deux dimensions. La séparation est effectuée au moyen de membranes polymères amphiphiles, de plaques de chromatographie sur couche mince amphiphiles ou autres surfaces amphiphiles planaires en tant que phase fixe, avec une combinaison de tampons organiques et/ou aqueux en tant que phase mobile. Une sélection systématique des supports de phase fixe, des tampons de phase mobile et des conditions de fonctionnement permet d'adapter cette invention à une vaste gamme d'applications en protéomique, en spectrométrie de masse, dans la découverte de médicaments et dans les sciences pharmaceutiques.

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