C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/54 (2006.01) C07K 14/475 (2006.01) C07K 14/705 (2006.01) C07K 14/71 (2006.01) C12N 9/12 (2006.01) C12Q 1/68 (2006.01) G01N 33/566 (2006.01) G01N 33/573 (2006.01) G01N 33/74 (2006.01)
Patent
CA 2100860
A novel expression cloning method for detection and identification of proteins capable of binding to tyrosine-phosphory- lated domains of receptor tyrosine kinases is based on the use of a novel probe. This probe comprises an amino acid sequence de- rived from the tyrosine-phosphorylated-portion of the receptor molecule, or a functional derivative thereof, and has at least one phosphorylated tyrosine residue, lacks the tyrosine kinase portion of the receptor, and is delectably labeled. Also disclosed are a method for preparing the probe, a method for mapping to a chromosome a gene encoding a protein capable of binding to tyro- sine-phosphorylated domains of receptor tyrosine kinases, and a method for purifying such a protein with the probe. Two pro- teins discovered using the above cloning method, GRB-1 and GRB-2, DNA encoding these proteins, and methods for detecting these proteins are also disclosed.
Margolis Benjamin L.
Schlessinger Joseph
Skolnik Edward Y.
Gowling Lafleur Henderson Llp
New York University
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