A - Human Necessities – 61 – K
Patent
A - Human Necessities
61
K
A61K 39/00 (2006.01) A61K 39/02 (2006.01) A61K 39/04 (2006.01) A61K 39/38 (2006.01) A61K 39/395 (2006.01) A61K 39/40 (2006.01) C12Q 1/00 (2006.01) G01N 33/53 (2006.01) G01N 33/531 (2006.01) G01N 33/532 (2006.01) G01N 33/533 (2006.01) G01N 33/542 (2006.01) G01N 33/566 (2006.01) G01N 33/569 (2006.01) G01N 33/58 (2006.01)
Patent
CA 2464473
The present invention provides an assay for detection of serum antibodies to M. paratuberculosis. A tracer, comprising a carbohydrate antigen isolated from M. paratuberculosis that is conjugated to a fluorophore, is added to a serum sample from an animal to form a mixture. The fluorescence polarization of the mixture is then measured. The presence of serum antibodies toM. paratuberculosis is indicated by a fluorescence polarization value of the mixture that is higher than the fluorescence polarization value of a control. The present invention further provides a tracer for use in a fluorescence polarization assay for antibodies specific for M. paratuberculosis. The tracer comprises a carbohydrate antigen isolated from M. paratuberculosis and conjugated to a fluorophore, such that the tracer is able to bind to antibodies specific for M. paratuberculosis to produce a detectable change in fluorescence polarization.
Cette invention a trait à un dosage servant à la détection d'anticorps sériques contre le mycobacterium de la paratuberculose. On ajoute un indicateur traceur, renfermant un antigène carbohydrate isolé dudit mycobacterium et conjugué à un fluorophore, à un prélèvement sérique issu d'un animal et ce, afin de constituer un mélange. On mesure alors la polarisation de fluorescence du mélange. La présence d'anticorps sériques contre le mycobacterium de la paratuberculose est signalée par une valeur de polarisation de fluorescence du mélange supérieure à celle d'un échantillon témoin. L'invention porte également sur un indicateur traceur utilisable dans un dosage de polarisation de fluorescence relatif à des anticorps spécifiques du mycobacterium de la paratuberculose. Cet indicateur traceur, qui renferme un antigène carbohydrate isolé dudit mycobacterium et conjugué à un fluorophore, est, de la sorte, en mesure de se fixer à des anticorps spécifiques du mycobacterium de la paratuberculose et ce, de provoquer une modification décelable de la polarisation de fluorescence.
Stilwell Kathryn Irene
Surujballi Om P.
Diachemix Llc
Her Majesty The Queen In Right Of Canada As Represented By The C
Mbm Intellectual Property Law Llp
LandOfFree
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