Method of selecting specific bacteriophages

Details Method of selecting specific bacteriophages Method of selecting specific bacteriophages

C - Chemistry, Metallurgy

12

N

C12N 15/64 (2006.01) C12N 7/01 (2006.01) C12N 15/10 (2006.01) G01N 33/554 (2006.01)

Patent

CA 2178205

A method for selecting a molecule, such as an antibody, antigen, peptide. protein or fragment thereof, which molecule is expressed together with a phage coat protein on the phage's surface. The method is characterised by linking phage replication to recognition of the molecule on the surface of the phage. The linkage can be achieved by use of a fusion protein between phage protein 3 and a specific binding ligand for the molecule.

Un procédé permet de sélectionner une molécule, tel qu'un anticorps, un antigène, un peptide, une protéine ou un de leurs fragments, molécule qui est exprimée avec une protéine d'enveloppe de phage placée à la surface de ce dernier. Ce procédé se caractérise par l'établissement d'un lien entre la réplication d'un phage et la reconnaissance de la molécule placée à la surface de ce dernier. Ce lien peut s'obtenir en utilisant une protéine de fusion entre la protéine 3 du phage et un ligand de liaison spécifique de cette molécule.

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