Peptide libraries as a source of syngenes

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/62 (2006.01) A61K 31/70 (2006.01) A61K 38/03 (2006.01) A61K 38/16 (2006.01) A61K 48/00 (2006.01) C07K 14/00 (2006.01) C07K 14/47 (2006.01) C12N 15/10 (2006.01) C12N 15/11 (2006.01) C12N 15/85 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2197864

The present invention relates generally to synthetic gene sequences ("syngenes") that are identified by screening random peptide librairies for peptides that bind a ligand of choice. The synthetic gene sequences, together with, optionally, other DNA sequences that target the synthetic gene sequences or their encoded proteins to particular locations in vivo or intracellularly, or that contain processing signals, or that code for other peptides or amino acid sequences, are cloned into suitable expression vectors. The syngenes are used, for example, in gene therapy to supply, via expression of their encoded proteins, a therapeutic product. In another aspect, the invention relates to protein or peptide products of syngenes and their therapeutic and diagnsotic uses.

La présente invention concerne d'une manière générale des séquences de gènes synthétiques (syngènes) que l'on identifie en recherchant dans des banques de peptides aléatoires, des peptides qui se fixent au ligand choisi. On clone dans des vecteurs appropriés les séquences de gènes synthétiques avec, éventuellement, d'autres séquences d'ADN, qui ciblent les séquences de gènes synthétiques ou leurs protéines codées sur des emplacements spécifiques in vivo ou intracellulaires, qui contiennent des signaux de traitement ou qui codent pour d'autres peptides ou séquences d'acides aminés. Les syngènes sont utilisés, par exemple, en thérapie génique pour fournir un produit thérapeutique, par le biais des protéines codées. Selon un autre aspect, l'invention concerne des produits protéiques ou peptidiques de syngènes et leurs utilisations à des fins thérapeutiques et diagnostiques.

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