Self-calibration system for a magnetic binding assay

G - Physics – 01 – N

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G01N 33/543 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2495075

A self-calibrated, magnetic binding assay (e.g., sandwich, competitive, etc.) for detecting the presence or quantity of an analyte residing in a test sample is provided. The magnetic binding assay includes detection probes capable of generating a detection signal (e.g., fluorescent non-magnetic particles) and calibration probes capable of generating calibration signal (e.g., fluorescent magnetic particles). The amount of the analyte within the test sample is proportional to the intensity of the detection signal calibrated by the intensity of the calibration signal.

L'invention concerne un dosage de liaison magnétique auto-étalonné (par exemple, sandwich, compétitif, etc) permettant de détecter la présence ou la quantité d'un analyte renfermé dans un échantillon test. Ce dosage de liaison magnétique comprend des sondes de détection permettant de générer un signal de détection (par exemple, des particules non magnétiques fluorescentes) et des sondes d'étalonnage permettant de générer un signal d'étalonnage (par exemple, des particules magnétiques fluorescentes). La quantité d'analyte renfermée à l'intérieur de l'échantillon test est proportionnelle à l'intensité du signal de détection étalonné par l'intensité du signal d'étalonnage.

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