Method for the detection of hpv and probes, primers and kits

Details Method for the detection of hpv and probes, primers and kits Method for the detection of hpv and probes, primers and kits

C - Chemistry, Metallurgy

12

Q

C12Q 1/70 (2006.01)

Patent

CA 2595390

The invention relates to materials and methods method for detection and/or typing of any HPV nucleic acid possibly present in a biological sample, the method comprising the steps of:(i) amplification of a polynucleic acid fragment comprising or consisting of the B region of any HPV nucleic acid in the sample, said B region being indicated in Figure 1, and (ii) contacting any amplified fragments from step (i) with at least one probe capable of specific hybridization with the B region of HPV, said B region being indicated in Figure 1.

L'invention concerne des matières et des méthodes pour la détection et/ou le typage d'un quelconque acide nucléique de VPH susceptible d'être présent dans un échantillon biologique. Cette méthode consiste (i) à réaliser l'amplification d'un fragment d'acide polynucléique comprenant la région B d'un quelconque acide nucléique de VPH dans l'échantillon, cette région B étant indiquée dans la Figure 1, et (ii) à mettre des fragments amplifiés de l'étape (i) en contact avec au moins une sonde pouvant subir une hybridation spécifique avec la région B du VPH, ladite région B étant indiquée dans la Figure 1.

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