Methods for testing of therapeutic compounds

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/70 (2006.01) A61K 49/00 (2006.01) C12Q 1/00 (2006.01) G01N 33/15 (2006.01)

Patent

CA 2211414

The present invention is related to a method for determining the efficacy of a chemical compound for treatment of recurrent herpes virus infection, said method comprising: (a) inoculating herpes virus at a primary skin site of a non-human mammal to cause zosteriform spread of the virus through nerve cells to cause lesions at a secondary (distal) site of infection; (b) before the appearance of skin lesions at the secondary site caused by the virus, giving adoptive transfer of immunity (ATI) to the said non-human mammal, so as to provide immunity mimicking the immunological reactivity seen in a recurrent herpes virus infection in a previously infected immune host; (c) administering a chemical compound to be tested to the said non-human mammal; and (d) determining the ability of the said chemical compound to reduce the occurrence and/or severity of herpes virus-induced skin lesions at the secondary site of infection.

La présente invention concerne un procédé permettant de déterminer l'efficacité d'un composé chimique destiné au traitement de l'infection récurrente au virus de l'herpès. Ce procédé consiste: a) à inoculer le virus de l'herpès au niveau d'un site cutané primaire d'un mammifère non humain de façon à favoriser la propagation zoniforme du virus par les cellules nerveuses jusqu'à provoquer des lésions au niveau d'un site secondaire d'infection (distal); b) à conférer audit mammifère non humain, avant l'apparition des lésions cutanées provoquées par le virus au niveau du site secondaire, une immunité de transfert adoptive (ATI) de façon à procurer une immunité imitant la réactivité immunologique constatée dans une infection récurrente au virus de l'herpès chez un hôte immun préalablement infecté; c) à administrer audit mammifère non humain un composé chimique à tester; et d) à déterminer l'aptitude dudit composé chimique à réduire l'apparition et/ou la gravité des lésions cutanées induites par le virus de l'herpès au niveau du site secondaire d'infection.

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