Fluorescence detection system

Details Fluorescence detection system Fluorescence detection system

G - Physics

01

N

G01N 21/64 (2006.01)

Patent

CA 2593814

Apparatus and methods for exciting and detecting fluorescence in samples are disclosed. In one embodiment, a sample holder for holding a plurality of samples is provided together with an optical manifold having an excitation source, a photo receiver, or both, for each of the plurality of samples. In another embodiment, the optical manifold contains only the excitation source or a photo receiver, and the other is associated with the sample holder. This system permits for rapid excitation and measurement of fluorescence without the use of moving parts and without any opto~mechanical or electronic disturbance. It exhibits an exceptional signal to noise ratio, which permits it to differentiate between very low level differences in fluorescence.

L'invention concerne des appareils et des procédés permettant d'exciter et de détecter la fluorescence dans des échantillons. Dans un mode de réalisation, l'invention concerne un porte-échantillon destiné à supporter une pluralité d'échantillons conjointement avec un collecteur optique comprenant une source d'excitation, un récepteur photo ou les deux à la fois pour chaque échantillon. Dans un autre mode de réalisation, le collecteur optique ne contient que la source d'excitation ou un récepteur photo tandis que l'autre élément est associé au porte-échantillon. Ce système permet de produire une excitation rapide et de mesurer la fluorescence sans avoir recours à des parties mobiles et sans perturbation opticomécanique ou électronique.

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