Immobilisation of antigenic carbohydrates to support...

G - Physics – 01 – N

Patent

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G01N 33/569 (2006.01) G01N 33/543 (2006.01)

Patent

CA 2605493

The invention relates to the field of chemistry and diagnosis, more in particular to diagnosis of current and/or past and/or symptomless infections or of a history of exposure to a gram-negative-bacterium (such as an enterobacteriaceae or a legionella). Even more in particular, the invention relates to the screening of animals or animal products for the presence of unwanted/undesired microorganisms. The invention further relates to a method for screening samples for the presence of antibodies directed against unwanted/undesired microorganisms and preferably such a method is performed with help of a biosensor. The invention also relates to a method for immobilising polysaccharides to solid surfaces. The invention furthermore provides solid surfaces with immobilised polysaccharides as well as applications of such surfaces.

L'invention se rapporte au domaine de la chimie et du diagnostic, notamment au diagnostic d'infections présentes et/ou passée et/ou sans symptôme ou à un historique de l'exposition à une bactérie gram-négatif (telle qu'une enterobacteriaceae ou une legionella). L'invention porte encore plus particulièrement sur le dosage de la présence de micro-organismes indésirables chez des animaux ou des produits animaux. Elle concerne encore une méthode de dosage de la présence d'anticorps dirigés contre des micro-organismes indésirables dans des échantillons, méthode de préférence mise en oeuvre à l'aide d'un biocapteur. Une méthode d'immobilisation de polysaccharides sur des surfaces solides, des surfaces solides à polysaccharides immobilisés ainsi que les applications desdites surfaces sont décrites.

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